Rabbit Anti-Mullerian Hormone (AMH) ELISA Kit from MyBioSource.com

Supplier Page

Supplier Page from
MyBioSource.com for
Rabbit Anti-Mullerian Hormone (AMH) ELISA Kit

Get Pricing

Description

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This AMH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit AMH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY AMH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AMH antibody and an AMH-HRP conjugate. The assay sample and buffer are incubated together with AMH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMH concentration since AMH from samples and AMH-HRP conjugate compete for the anti-AMH antibody binding site. Since the number of sites is limited, as more sites are occupied by AMH from the sample, fewer sites are left to bind AMH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMH concentration in each sample is interpolated from this standard curve